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Home / Primary Antibodies / Polyclonal Antibodies

Rabbit Anti-P. falciparum HSP70 (P. falciparum) Polyclonal (SPC-186)

BHA11907218
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Email
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Antigen HSP70
Swissprot P11144
Accession Number M19753
Target HSP70
Research Area Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Tumor Biomarkers, Protein Trafficking
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Purification Protein A Purified
Scientific Background HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (5). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. PfHSP70-I (PF08_0054) is the major cytosolic HSP70 in Plasmodium falciparum. It is abundantly expressed in the blood stages of the parasite and is thought to constitute 1-2% of total parasite protein. It is induced upon heat shock. It is present in the parasite in different complexes with PfHSP90 and some PfHSP40 (6, 7). Looking for more information on HSP70 Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~ 70kDa. Specific to P. falciparum and does not cross-react to any protein from Human erythrocytes.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications WB, ICC/IF
Description WB ; ICC ; IF
Dilution WB (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1) 1-17.

2. Rothman J. (1989) Cell 59: 591 -601.

3. DeLuca-Flaherty, et al. (1990) Cell. 62: 875-887.

4. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294.

5. Fink A.L. (1999) Physiol. Rev. 79: 425-449.

6. Pesce E.R., et al. (2008) Int J Biochem Cell Biol. 40(12): 2914-26.

7. Pavithra S.R, Banumathy G., Joy O., Singh V., Tatu U. (2004) J Biol Chem. 279(45): 46692-9.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11907218-1 SPC-186D Unconjugated 100 ug $337
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BHA11907218-2 SPC-186D-A390 ATTO 390 100 ug $392
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BHA11907218-3 SPC-186D-A488 ATTO 488 100 ug $391
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BHA11907218-4 SPC-186D-A594 ATTO 594 100 ug $391
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BHA11907218-5 SPC-186D-APC APC 100 ug $390
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BHA11907218-6 SPC-186D-BI Biotin 100 ug $386
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BHA11907218-7 SPC-186D-FITC FITC 100 ug $381
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BHA11907218-8 SPC-186D-HRP HRP 100 ug $377
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BHA11907218-9 SPC-186D-PCP PerCP 100 ug $390
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BHA11907218-10 SPC-186D-RPE RPE 100 ug $388
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BHA11907218-11 SPC-186S Unconjugated 12 ug $45
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