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Home / Primary Antibodies / Polyclonal Antibodies

Rabbit Anti-Human HO-1 Polyclonal (SPC-112)

BHA11906404
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Email
orders@biohippo.com
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Antigen HO-1
Swissprot P09601
Accession Number NP_002124.1
Gene Id 3162
Target HO-1
Research Area Cancer, Oxidative Stress, Neurodegeneration, Alzheimer's Disease, Cardiovascular System, Blood, Cell Signaling, Epigenetics and Nuclear Signaling, Nuclear Signaling Pathways, NFkB Pathway, Atherosclerosis, Vascular Inflammation, Inflammatory Mediators, Vasculature, Endothelium, Cancer Metabolism, Response to Hypoxia, Metabolism, Metabolism processes, Hypoxia, Neuroscience, Platelets
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein A Purified
Scientific Background Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2).
There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~32kDa.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, WB, ICC/IF
Description WB ; ICC ; IF ; IHC
Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86.

2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47.

3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419.

4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8.

5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930.

6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761.

7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906404-1 SPC-112D Unconjugated 100 ug $281
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BHA11906404-2 SPC-112D-A390 ATTO 390 100 ug $336
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BHA11906404-3 SPC-112D-A488 ATTO 488 100 ug $335
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BHA11906404-4 SPC-112D-A594 ATTO 594 100 ug $335
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BHA11906404-5 SPC-112D-APC APC 100 ug $334
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BHA11906404-6 SPC-112D-BI Biotin 100 ug $329
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BHA11906404-7 SPC-112D-FITC FITC 100 ug $325
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BHA11906404-8 SPC-112D-HRP HRP 100 ug $321
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BHA11906404-9 SPC-112D-PCP PerCP 100 ug $334
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BHA11906404-10 SPC-112D-RPE RPE 100 ug $332
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BHA11906404-11 SPC-112S Unconjugated 12 ug $45
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