Rat MMP-2 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0639

Sensitivity

<300 pg/ml

Detection Range

625 pg/ml - 40,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.029	0.115	0.173	0.310	0.537	0.976	1.555	2.153

Description

Rat MMP-2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Mmp2 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A30-C662

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Rat Mmp2

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2001	4516	23614	1093	4543	21263
Standard deviation	176.09	320.64	1157.09	86.35	367.98	1318.31
CV (%)	8.8%	7.1%	4.9%	7.9%	8.1%	6.2%

Background

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue tuover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	2001	1093	986	1110	1298	63.58	4.9%
Sample 2	4516	3756	4531	4695	4375	253.72	5.8%
Sample 3	23614	23697	22457	26546	24079	1396.55	5.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	81686
Gene Name	CST3
UniProt ID	P33436
Protein Name	72 kDa type IV collagenase

Applications	ELISA
Research Area	Cardiovascular,Caspases,Cell Biology,Cell Type Markers,Neural Stem Cells,Stem Cells,Tags & Cell Markers,Tumor Associated

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000236	FEK0639	96 wells/kit	$499		Add to Cart

Rat MMP-2 PicoKine® Quick ELISA Kit

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