Rat IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit

Mfr.No.

FEK0393

Sensitivity

<1 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.031	0.079	0.146	0.207	0.338	0.569	1.051	1.829

Description

Rat IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Il1b in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin 1 beta ;Interleukin-1 beta ;Il1b ;rCG_27107 ;

Molecular Weight

30659 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V117-S268

Kit Components

Description Quantity Volume
Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
HRP-Linked Antibody 1 6ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat IL-1 beta

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	58	233	962	62	241	881
Standard deviation	3.65	11.41	69.26	4.21	12.29	68.71
CV (%)	7.8%	4.9%	7.2%	6.8%	7.8%	7.8%

Background

The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response . Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone tuover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1 (VCAM-1) on hepatic sinusoidal endothelium (HSE).

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	58	59	57	56	57	1.11	1.9%
Sample 2	233	268	237	251	247	13.71	5.5%
Sample 3	962	826	929	964	920	56.16	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	24494
Gene Name	IL1B
UniProt ID	Q63264
Protein Name	Interleukin-1 beta

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001894	FEK0393	96 wells/kit	$399		Add to Cart

Rat IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit

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