Rat CXCL2/MIP-2 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0725

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1,000
O.D.	0.060	0.233	0.380	0.672	1.188	1.948	2.349	2.470

Description

Rat CXCL2/MIP-2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat CXCL2/MIP-2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S32-N100

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Rat Cxcl2

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	36	225	364	40	196	377
Standard deviation	1.58	10.58	14.2	2.2	10	21.11
CV(%)	4.4%	4.7%	3.9	5.5%	5.1%	5.6%

Background

MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	36	30	29	35	33	1.66	5.1%
Sample 2	225	212	196	180	203	9.96	4.9%
Sample 3	364	399	412	387	391	11.32	2.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	114105
Gene Name	Cxcl2
UniProt ID	P30348

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000286	FEK0725	96 wells/kit	$499		Add to Cart

Rat CXCL2/MIP-2 PicoKine® Quick ELISA Kit

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Reproducibility

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