Rat Adiponectin PicoKine® Quick ELISA Kit

Mfr.No.

FEK1327

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.047	0.153	0.227	0.387	0.690	1.252	2.000	2.397

Description

Rat Adiponectin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Adipoq in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

30 kDa adipocyte complement-related protein ;Adiponectin, C1Q and collagen domain containing ;Protein Adipoq ;Adipoq ;Acdc ;rCG_36676 ;

Molecular Weight

26410 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I19-N244

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Rat Adipoq

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	292	1705	4269	265	1626	4075
Standard deviation	16.93	126.17	170.76	19.61	133.33	167.07
CV (%)	5.8%	7.4%	4%	7.4%	8.2%	4.1%

Background

Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	292	307	293	277	292	10.61	3.6%
Sample 2	1705	1526	1807	1787	1706	110.86	6.4%
Sample 3	4269	4351	4667	4673	4490	182.33	4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	246253
Gene Name	Adipoq
UniProt ID	Q8K3R4
Protein Name	Adiponectin

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000145	FEK1327	96 wells/kit	$499		Add to Cart

Rat Adiponectin PicoKine® Quick ELISA Kit

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