Mouse PTX3/Pentraxin 3 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0862

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.035	0.119	0.209	0.344	0.626	1.028	1.542	2.207

Description

Mouse PTX3/Pentraxin 3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Ptx3 in cell culture supeatants, serum and plasma (EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Pentraxin-related protein PTX3;Pentaxin-related protein PTX3;Tumor necrosis factor-inducible gene 14 protein;TSG-14;Ptx3;Tsg14;

Molecular Weight

42 kDa

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-S381

Kit Components

Description Quantity Volume
Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
HRP-Linked Antibody 1 6ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse PTX3

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	262	1334	4146	264	1451	3917
Standard deviation	15.19	98.71	211.44	15.84	113.17	274.19
CV (%)	5.8%	7.4%	7%	6%	7.8%	7%

Background

PTX3 (Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	262	259	249	272	260	8.2	3.1%
Sample 2	1334	1350	1444	1511	1409	71.99	5.1%
Sample 3	4146	4596	4000	4523	4316	250	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	19288
Gene Name	PTX3
UniProt ID	P48759
Protein Name	Pentraxin-related protein PTX3

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001898	FEK0862	96 wells/kit	$499		Add to Cart

Mouse PTX3/Pentraxin 3 PicoKine® Quick ELISA Kit

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