Mouse IgG (Immunoglobulin G) ELISA Kit

Mfr.No.

E-OSEL-M0005

Sensitivity

0.51 ng/mL

Detection Range

1.56-100 ng/mL

Reactivity

Mouse

Assay Duration

1.5h

Assay Type

Sandwich

Description

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG are added to the micro ELISA plate wells. Mouse IgG in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IgG. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names

Immunoglobulin G,IgG

Detection Method

Colormetric

Kit Components

An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells x12 strips 48T: 8 wells x6 strips 24T: 8 wells x3 strips 96T*5: 5 plates, 96T	2-8°C, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100x)	96T: 1 vial, 60 uL 48T/24T: 1 vial, 30 uL 96T*5: 5 vials, 60 uL	2-8°C(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8°C
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25x)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8°C(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8°C
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Recovery

80%-120%. The recovery of IgG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-101	93
EDTA plasma(n=8)	92-107	99

Specificity

This kit recognizes IgG in samples. No significant cross-reactivity or interference between IgG and analogues was observed.

Precision

Both intra-CV and inter-CV are < 10%.
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IgG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IgG were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	5.07	8.36	48.61	5.40	9.15	48.67
Standard deviation	0.29	0.44	2.38	0.35	0.46	1.65
CV (%)	5.77	5.28	4.90	6.46	5.03	3.38

Usage Notes

This ELISA kit applies to the in vitro quantitative determination of Mouse IgG concentrations in serum, plasma.

Applications	ELISA
Applications Notes	This ELISA kit applies to the in vitro quantitative determination of IgG concentrations in serum and plasma.
Research Area	Immunology

Storage	An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual., 2-8°C,6 months
Shipping	Ice bag

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SKU	Supplier No.	Size	Your price	Quantity
BHE10308159	E-OSEL-M0005	96T	$520		Add to Cart
BHE10308159	E-OSEL-M0005	48T	$416		Add to Cart

Mouse IgG (Immunoglobulin G) ELISA Kit

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