Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit

Mfr.No.

FEK1313

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1,000	2,000
O.D.	0.073	0.167	0.225	0.369	0.639	1.107	1.793	2.400

Description

Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T40-R217

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Mouse Fcgr2b/Fcgr3

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	88	208	904	96	234	841
Standard deviation	3.96	12.9	29.83	5.38	14.27	58.03
CV (%)	4.5%	6.2%	3.3%	5.6%	6.1%	6.9%

Background

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	88	87	94	85	89	4.69	5.3%
Sample 2	208	235	216	244	226	11.06	4.9%
Sample 3	904	867	905	841	879	39.57	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	14130, 14131
Gene Name	Fcgr2b/Fcgr3
UniProt ID	P08101, P08508

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000317	FEK1313	96 wells/kit	$499		Add to Cart

Mouse Fc Gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit

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Reproducibility

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