Mouse CTLA4 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0717

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1,000	2,000
O.D.	0.016	0.129	0.228	0.392	0.726	1.301	1.978	2.589

Description

Mouse CTLA4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse CTLA4 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A37-F162

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Mouse Ctla4

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	88	302	874	79	274	826
Standard deviation	4.84	20.84	41.95	5.21	16.17	58.65
CV(%)	5.5%	6.9%	4.8%	6.6%	5.9%	7.1%

Background

CTLA-4(Cytotoxic T-Lymphocyte Antigen 4), also known as CD152(Cluster of differentiation 152), is a protein receptor that down regulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	88	91	95	99	93	3.08	3.3%
Sample 2	302	299	240	274	279	13.66	4.9%
Sample 3	874	726	864	791	814	42.32	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	12477
Gene Name	Ctla4
UniProt ID	P09793

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000296	FEK0717	96 wells/kit	$499		Add to Cart

Mouse CTLA4 PicoKine® Quick ELISA Kit

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Reproducibility

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