Mouse CCL25/TECK PicoKine® Quick ELISA Kit

Mfr.No.

FEK1424

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1,000	2,000
O.D.	0.021	0.105	0.161	0.289	0.557	1.003	1.685	2.351

Description

Mouse CCL25/TECK PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse CCL25/TECK in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-N144

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Mouse Ccl25

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	92	364	941	97	276	984
Standard deviation	4.97	17.42	70.58	5.92	20.7	66.91
CV (%)	5.4%	6.6%	7.5%	6.1%	7.5%	6.8%

Background

Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed Chemokine). The mouse Teck gene was mapped to chromosome 8 and human Teck gene was to 19p13.2. CCL25 is believed to play a role in the development of T-cells. It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 elicits its effects by binding to the chemokine receptor CCR9.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	92	98	86	97	93	3.08	3.3%
Sample 2	264	284	257	266	268	18.47	6.9%
Sample 3	941	985	981	953	965	27.99	2.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	20300
Gene Name	Ccl25
UniProt ID	O35903

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000312	FEK1424	96 wells/kit	$499		Add to Cart

Mouse CCL25/TECK PicoKine® Quick ELISA Kit

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Reproducibility

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