Mouse Adiponectin PicoKine® Quick ELISA Kit

Mfr.No.

FEK0596

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.021	0.068	0.126	0.393	0.623	1.039	1.452	2.063

Description

Mouse Adiponectin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Adipoq in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Adiponectin;30 kDa adipocyte complement-related protein;Adipocyte complement-related 30 kDa protein;ACRP30;Adipocyte, C1q and collagen domain-containing protein;Adipocyte-specific protein AdipoQ;Adipoq;Acdc, Acrp30, Apm1;

Molecular Weight

26809 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-N244

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant full length Mouse Adipoq

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	567	2994	7572	512	3121	7518
Standard deviation	37.98	179.64	318.02	37.75	240.31	330.79
CV (%)	6.7%	6%	4.2%	7.1%	7.7%	4.4%

Protein Function

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. .

Background

Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	567	603	628	571	592	24.91	4.2%
Sample 2	2994	2867	2845	2816	2880	67.97	2.3%
Sample 3	7572	7510	8167	7413	7665	295.03	3.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	11450
Gene Name	Adipoq
UniProt ID	Q60994
Protein Name	Adiponectin
Tissue Specificity	Synthesized exclusively by adipocytes and secreted into plasma.
Sequence	Contains 1 C1q domain.

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000144	FEK0596	96 wells/kit	$499		Add to Cart

Mouse Adiponectin PicoKine® Quick ELISA Kit

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