Human TIMP-2 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0522

Sensitivity

<2 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.005	0.109	0.198	0.418	0.787	1.397	2.109	2.513

Description

Human TIMP-2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human TIMP2 in cell culture supeatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Metalloproteinase inhibitor 2;CSC-21K;Tissue inhibitor of metalloproteinases 2;TIMP-2;TIMP2;

Molecular Weight

24399 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: C27-P220

Kit Components

Description Quantity
96-well plate 1
lyophilized Standard
Biotinylated detection antibody (50x) 100 ul
Avidin-Biotin-Peroxidase Complex (30x) 400 µl
Sample diluent 30 ml
Antibody diluent 12 ml
Avidin-Biotin-Peroxidase Diluent 12 ml
Wash Buffer (25x) 20 ml
Color Developing Reagent (TMB) 10 ml
Stop Solution 10 ml
Plate Sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TIMP-2

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	249	1786	4399	239	1654	4356
Standard deviation	17.4%3	94.65	325.52	16.73	112.47	361.54
CV (%)	6.4%	5.3%	7.4%	7%	6.8%	8.3%

Protein Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. .

Background

TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	249	258	275	291	268	16.11	6%
Sample 2	1786	1798	1796	2014	1848	95.65	5.1%
Sample 3	4399	5066	4506	5016	4746	297.19	6.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7077
Gene Name	TIMP2
UniProt ID	P16035
Protein Name	Metalloproteinase inhibitor 2
Sequence	Belongs to the protease inhibitor I35 (TIMP) family.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000169	FEK0522	96 wells/kit	$499		Add to Cart

Human TIMP-2 PicoKine® Quick ELISA Kit

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