Human IL-15/Interleukin-15 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0426

Sensitivity

<3 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.010	0.112	0.180	0.283	0.668	1.056	1.885	2.523

Description

Human IL-15 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human IL15 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Interleukin-15;IL-15;IL15;

Molecular Weight

18086 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N49-S162

Kit Components

Description Quantity
96-well plate 1
lyophilized Standard
Biotinylated detection antibody (50x) 100 ul
Avidin-Biotin-Peroxidase Complex (30x) 400 µl
Sample diluent 30 ml
Antibody diluent 12 ml
Avidin-Biotin-Peroxidase Diluent 12 ml
Wash Buffer (25x) 20 ml
Color Developing Reagent (TMB) 10 ml
Stop Solution 10 ml
Plate Sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human IL-15

Subcellular Localization

Isoform IL15-S48AA: Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	35	140	427	32	143	390
Standard deviation	2.34	6.16	19.64	2.52	8.72	23.79
CV (%)	6.7%	4.4%	4.6%	7.9%	6.1%	6.1%

Protein Function

Cytokine that stimulates the proliferation of T- lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.

Background

Interleukin (IL)-15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2 (IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor. IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might thus be beneficial in the prevention of acute, and especially chronic, allograft rejection. The human IL15 gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization.1 The standard product used in this kit is recombinant human IL-15, consisting of 114 amino acids with the molecular mass of 12.9KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	35	37	37	35	36	1	2.7%
Sample 2	140	151	160	164	153	9.22	6%
Sample 3	427	395	364	407	398	22.84	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3600
Gene Name	IL15
UniProt ID	P40933
Protein Name	Interleukin-15
Tissue Specificity	Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus.
Sequence	Belongs to the IL-15/IL-21 family.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000166	FEK0426	96 wells/kit	$499		Add to Cart

Human IL-15/Interleukin-15 PicoKine® Quick ELISA Kit

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