Human IL-12(p70) PicoKine® Quick ELISA Kit

Mfr.No.

FEK0421

Sensitivity

<2 pg/ml

Detection Range

7.8 pg/ml - 500 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.021	0.088	0.140	0.274	0.513	0.953	1.620	2.044

Description

Human IL-12(P70) PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate human IL12A in cell culture supeatants and serum. Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: (I23-S328) + (R23-S219)

Kit Components

Description Quantity Volume
Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
HRP-Linked Antibody 1 6ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	18	51	294	19	55	310
Standard deviation	1.31	3.41	17.38	1.67	3.79	21.39
CV (%)	7.3%	6.7%	5.2%	8.8%	6.9%	6.9%

Background

Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response.(i) And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.(ii) Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.(iii) The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	18	18	20	17	18	1.08	6%
Sample 2	51	59	60	54	56	3.67	6.5%
Sample 3	294	290	284	296	291	4.58	1.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	3592
Gene Name	IL12A
UniProt ID	P29459

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001840	FEK0421	96 wells/kit	$499		Add to Cart

Human IL-12(p70) PicoKine® Quick ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human IL-12(p70) PicoKine® Quick ELISA Kit

Rate it now..!!