Human CHRDL1 PicoKine® Quick ELISA Kit

Mfr.No.

FEK1953

Sensitivity

<50 pg/ml

Detection Range

93.6 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.088	0.134	0.176	0.265	0.402	0.702	1.263	2.099

Description

Human CHRDL1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CHRDL1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Chordin-like protein 1; Neuralin-1; Neurogenesin-1; Ventroptin; CHRDL1; NRLN1

Molecular Weight

52 KDa

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-C450

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CHRDL1

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	146	511	1796	201	618	2851
Standard deviation	7.59	27.08	100.58	13.07	41.41	196.72
CV (%)	5.2%	5.3%	5.6%	6.5%	6.7%	6.9%

Background

Chordin-like 1 is a protein that in humans is encoded by the CHRDL1 gene. It is mapped to Xq23. This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alteatively spliced transcript variants encoding different isoforms have been described.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	146	138	126	133	135	7.29	5.4%
Sample 2	511	424	442	453	457	32.57	7.1%
Sample 3	1796	1945	1901	2041	1920	88.03	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	91851
Gene Name	CHRDL1
UniProt ID	Q9BU40
Protein Name	chordin like 1

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001901	FEK1953	96 wells/kit	$499		Add to Cart

Human CHRDL1 PicoKine® Quick ELISA Kit

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