Human BAFF/TNFSF13B PicoKine® Quick ELISA Kit

Mfr.No.

FEK0663

Sensitivity

<2 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	62.5	125	250	500	1,000	2,000	4,000
O.D.	0.018	0.088	0.119	0.223	0.391	0.716	1.262	1.941

Description

Human BAFF/TNFSF13B PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human BAFF/TNFSF13B in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A134-L285

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Human TNFSF13B

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	125	539	1336	125	530	1452
Standard deviation	6.12	40.42	78.82	6.00	43.99	110.35
CV(%)	4.9%	7.5%	5.9%	4.8%	8.3%	7.6%

Background

BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system(CNS) lymphomas.1 The TNF superfamily member B cell-activating factor(BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology.2 The B cell activating factor BAFF(BlyS/TALL-1/zTNF4) is a tumor necrosis factor(TNF)-related ligand that promotes B cell survival and binds to three receptors(BCMA, TACI, and the recently described BAFF-R).3 Human BAFF was mapped to chromosome 13q32-34.4 The standard used in this kit is recombinant soluble human BAFF(A134-L295) with the molecular mass of 19.6KDa.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	125	127	151	129	133	9.97	7.5%
Sample 2	539	515	538	530	530	21.73	4.1%
Sample 3	1336	1087	1217	1225	1216	79.04	6.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	10673
Gene Name	TNFSF13B
UniProt ID	Q9Y275

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000266	FEK0663	96 wells/kit	$499		Add to Cart

Human BAFF/TNFSF13B PicoKine® Quick ELISA Kit

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Reproducibility

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