Human APOA1/Apolipoprotein A-I PicoKine® Quick ELISA Kit

Mfr.No.

FEK1456

Sensitivity

<50 pg/ml

Detection Range

3.12 ng/ml - 200 ng/ml

Reactivity

Human

Sample Volume

Concentration(ng/ml)	0	3.12	6.25	12.5	25	50	100	200
O.D.	0.021	0.157	0.271	0.483	0.814	1.311	1.870	2.285

Description

Human APOA1/Apolipoprotein A-I PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human APOA1/Apolipoprotein A-I in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D25-Q267

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Human APOA1

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	8188	19829	93784	8185	19184	85441
Standard deviation	368.46	1249.22	5533.25	532.02	1477.16	5382.78
CV(%)	4.5%	6.3%	5.9%	6.5%	7.7%	6.3%

Background

Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high absorbance lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alteative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	8188	7998	8004	7332	7880	330.96	4.2%
Sample 2	19829	19750	20658	19659	19974	619.19	3.1%
Sample 3	93784	86245	87457	88574	89015	4005.67	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	335
Gene Name	APOA1
UniProt ID	P02647

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000269	FEK1456	96 wells/kit	$499		Add to Cart

Human APOA1/Apolipoprotein A-I PicoKine® Quick ELISA Kit

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Reproducibility

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