Hieff Unicon^TM Pro Universal TaqMan qPCR Mix(UDG Plus)

Mfr.No.

16710ES06, 16710ES03

Description

Hieff Unicon^TM Pro Universal TaqMan qPCR Master Mix (UDG plus) is a fluorescent quantitative qPCR reagent that uses the company's next-generation antibody method to heat start Taq enzyme. The 2x TaqMan qPCR Mix pre mixed reagent includes reaction buffer and enzyme mix, already containing Mg2+ and dNTP, and adding factors that effectively inhibit non-specific PCR amplification and improve the efficiency of multiple qPCR reactions, which can ensure primer efficiency while conducting up to four reactions. This product contains UDG enzyme, which can effectively prevent the risk of aerosol pollution.

Product Components

Components No.	Name	16710ES60	16710ES80	16710ES92	16710ES94
		100T	1,000T	10,000T	20,000T
16710	2×TaqMan qPCR Mix	1.25 mL	12.5 mL	125 mL	250 mL

Product Details

1.Reaction System

Components	Volume (μL)	Final Concentration
2×TaqMan qPCR mix	12.5	1×
Primer mix (10 μmol/L)	x	0.1-0.5 μmol/L
Probe mix (10 μmol/L)	x	50-250 nmol/L
Rox reference dye	0.5	1×
Template DNA/cDNA	10-Jan	-
ddH2O	up to 25	-

*The primer concentration can be adjusted appropriately according to the experimental requirements.

2. Reaction program

Cycle step	Temp.	Time	Cycles
UDG treatment	37℃	5 min	1
Initial denaturation	95℃	5 min	1
Denaturation	95℃	15 s	45
Annealing/Extension	60℃	30 s	45

*The annealing / extension temperature can be adjusted appropriately according to the experimental requirements.

Notes
Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety！

Specifications

Hot Start	Built-in hot start
Detection method	Primer-probe detection
PCR method	qPCR, One-Tube
Polymerase	Taq DNA polymerase
Type of sample	DNA

Applications

Probe-Based qPCR

Shipping	The product is shipped with dry ice.
Storage	Stored at -15℃ ~ -25℃ for 1 year.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHR20800016-1	16710ES06	1000 T	$395		Add to Cart
BHR20800016-2	16710ES03	100 T	$50		Add to Cart

Hieff Unicon^TM Pro Universal TaqMan qPCR Mix(UDG Plus)

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