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Home / Proteins & Peptide

Wee1, GST-Th-Tag

SKU : BHP18200238

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Mfr.No.	40412
Immunogen Region	215-646 (end)
Tag	N-terminal GST-Thrombin-Tag
Description	Human Wee1 protein, also known as Wee1A Kinase, GenBank Accession No. NM_003390, amino acids 215-646 (end), with an N-terminal GST-Thrombin tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 75 kDa. Host cell line: Sf9 cells Specific activity: >=42 pmol/min/ug
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 2mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was done in Kinase buffer containing 1 mM DTT using Poly-(Lys4:Tyr) substrate (0.2 mg/ml) and 20 uM ATP. Reaction was done at 30°C for 45 min.Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega) Avoid freeze/thaw cycles.
GenBank	NM_003390
Mw(kda)	75 kDa

Synonyms	Wee1A Kinase, Wee1 Protein
Uniprot	P30291

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.

Reference	1. Parker, L., et al., Science. 1992; 257(5078):1955.2. Lundgreen, K, et al., Cell. 1991 Mar; 64(6):1111-1122.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200238	40412	10 ug	$412.50		Add to Cart

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