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Home / Proteins & Peptide

VHL/CUL2/ELOB/ELOC/RBX1 Complex

SKU : BHP18201150

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BPS Bioscience

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Mfr.No.	100373-1
Immunogen Region	VHL: 2-212(end); CUL2: 2-745(end); ELOB: 2-118; ELOC: 2-112(end); Rbx1: 2-108(end)
Tag	VHL: N-terminal FLAG-tag; CUL2: N-terminal FLAG-tag; ELOB: N-terminal FLAG-tag; ELOC: N-terminal His-tag; Rbx1: N-terminal His-tag
Description	VHL/CUL2/ELOB/ELOC/RBX1 complex of human VHL (GenBank Accession No. NM_000551) a.a. 2-212(end) with N-terminal FLAG-tag and MW= 25 kDa, human CUL2 (GenBank Accession No. NM_003591), a.a. 2-745(end) with N-terminal FLAG-tag and MW= 88 kDa, human ELOB (GenBank Accession No. NM_007108), a.a. 2-118 with N-terminal FLAG-tag and MW= 14 kDa, ELOC (GenBank Accession No. NM_005648), a.a. 2-112(end) with N-terminal His-tag and MW= 13 kDa human Rbx1 (GenBank Accession No. NM_014248), a.a. 2-108(end) with N-terminal His-tag and MW= 13 kDa, individually expressed in a HEK293 expression system.
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT.
Species	Human
Application	Useful for the study of protein degradation and PROTAC design, and selectivity profiling.
Notes	ARV-771-mediated binding of VHL BPS #100373 to BET bromodomain was assessed using A-screen technology. Various amounts of VHL were incubated in 10-ul reaction with ARV-771 and GSTBRD3(BD2) BPS #31033 at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts. Avoid freeze/thaw cycles.
GenBank	VHL: NM_000551; CUL2: NM_003591; ELOB: NM_007108; ELOC: NM_005648; Rbx1: NM_005648
Mw(kda)	VHL: 25 kDa; CUL2: 88 kDa; ELOB: 14 kDa; ELOC: 13 kDa; Rbx1: 13 kDa

Synonyms	VHL, CUL2, ELOB, ELOC, RBX1
Uniprot	VHL: P40337; CUL2: Q13617; ELOB: Q15370; ELOC: Q15369; Rbx1: P62877

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Kamura, T., et al., Science. 1999; 284(5414): 657-661. 2. Stebbins, C.E., et al., Science. 1999; 284(5413): 455-461.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201150	100373-1	10 ug	$236.50		Add to Cart

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