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Home / Proteins & Peptide

USP5 (IsoT), FLAG-tag

SKU : BHP18200819

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BPS Bioscience

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Mfr.No.	80355
Description	Human USP5 (Ubiquitin-Specific Protease 5) or Isopeptidase T (IsoT), GenBank Accession No. NM_001098536, a.a. 2-858(end) with an N-terminal FLAG-tag, MW=96 kDa, expressed in a baculovirus-infected Sf9 cell expression system. Specific activity: 14.3 pmole/min/ug
Immunogen Region	2-858(end)
Tag	N-terminal FLAG-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 3 mM DTT, and 90 g/ml FLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP5 in a 50 uL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm. Avoid freeze/thaw cycles.
GenBank	NM_001098536
Mw(kda)	96 kDa

Synonyms	USP 5, Ubiquitin-Specific Protease 5, Isopeptidase T, IsoT, USP-5
Uniprot	P45974

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Dayal, S., et al. (2009). J. Biol. Chem. 284(8):5030-41. 2. Reyes-Turcu, F.E., et al. (2008). J. Biol. Chem. 283(28):19581-92.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200819	80355	50 ug	$467.50		Add to Cart

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