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Home / Proteins & Peptide

USP10, FLAG-tag

SKU : BHP18200821

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Mfr.No.	80360
Description	Human USP10 (Ubiquitin-Specific Protease 10) or Ubiquitin C-terminal Hydrolase 10, GenBank Accession No. NM_005153, a.a. 2-798(end) with an N-terminal FLAG-tag, MW=88 kDa, expressed in a baculovirus-infected Sf9 cell expression system. Specific activity: 1.5 pmole/min/ug
Immunogen Region	2-798(end)
Tag	N-terminal FLAG-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ug/ml FLAG peptide, 10% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP10 in a 50 uL volume. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm. Avoid freeze/thaw cycles.
GenBank	NM_005153
Mw(kda)	88 kDa

Synonyms	USP 10, Ubiquitin-Specific Protease 10, Ubiquitin C-terminal Hydrolase 10, Ubch10, USP-10
Uniprot	Q14694

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Faus, H., et al. (2005). Mol. Cell. Endocrinol. 245(1-2):138-46. 2. Soncini, C., et al. (2001). Oncogene 20(29):3869-79. 3. Yuan, J., et al. (2010). Cell 140(3): 384-96.Application Reference: Oi, N., et al. Oncogene. 2015 May 14;34(20):2660-71. Resveratrol induces apoptosis by directly targeting Ras-GTPase-activating protein SH3 domain-binding protein 1

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200821	80360	50 ug	$467.50		Add to Cart

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