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Home / Proteins & Peptide

Uracil DNA Glycosylase

BHP11001174
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Antigen UNG
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Buffer 10X UNG Reaction Buffer: 200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA.
Description Formulation: UNG solution in 10mM Tris-HCl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol.
Specific Activity: The Specific Activity was found to be 5U/µl.
Unit Definition: 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C.
Inactivation: Inactivated by heating at 95°C for 10min. Enzyme activity is partially restored at temperatures lower than 55°C.
Reaction Conditions: 1X UNG Reaction Buffer, incubate at 37°C.UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.
Format Sterile Filtered colorless solution.
Expiry Date Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored below -18°C. Please prevent freeze-thaw cycles.
SKU Supplier No. Size Your price Quantity
BHP11001174 enz-352 2 $200
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BHP11001174 enz-352 $200
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BHP11001174 enz-352 10 $200
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BHP11001174 enz-352 $800
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BHP11001174 enz-352 20 $200
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BHP11001174 enz-352 $1400
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