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Home / Proteins & Peptide

UCHL3, His-tag

SKU : BHP18200818

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BPS Bioscience

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Mfr.No.	80353
Description	UCHL3, also known as Ubiquitin Carboxy-terminal Hydrolase L3 (GenBank Accession No. NM_006002), a.a. 2-231(end), with N-terminal His-tag, MW=27.1 kDa, expressed in E. coli. Specific activity: 20,733 pmole/min/ug
Immunogen Region	2-231(end)
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and UCHL3 in a 50 uL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation 340 nm, emission = 460 nm. Avoid freeze/thaw cycles.
GenBank	NM_006002
Mw(kda)	27.1 kDa

Synonyms	UCHL3, Ubiquitin Carboxy-terminal Hydrolase L3, Ubiquitin C-terminal Hydrolase L3
Uniprot	P15374

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Wada, H., et al. (1998). Biochem. Biophys. Res. Commun. 251 (3): 688-92. 2. Nam, M.J., et al. (2004). Proteomics 3 (11): 2108-15. 3. Setsuie, R., et al. (2009). Neurochem Int. 54(5-6):314-21.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200818	80353	100 ug	$401.50		Add to Cart

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