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Home / Proteins & Peptide

TNF-alpha (mutant), Human

SKU : BHP18500006

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Mfr.No.	IX00404, IX00404-1, IX00404-10, IX00404-50
Description	TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF. The clinical use of the potent anti-tumor activity of TNF-alpha has been limited by the proinflammatory side effects including fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-a mutants with low systemic toxicity has been an intense pharmacological interest. Human TNF-a, which binds to the murine TNF-R55 but not to the mouse TNF-R75, exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-a, which binds to both murine TNF receptors. Based on these results, many TNF-a mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro, and exhibited lower systemic toxicity in vivo. Recombinant Human TNF-alpha Variant/Mutant compared with the wild-type, has an amino acid sequence deletion from a.a. 1-7, and the following a.a. substitutes Arg8, Lys9, Arg10 and Phe157 which is proven to have more activity and with less inflammatory side effect in vivo. Recombinant Human TNF-alpha Mutant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 151 amino acids and having a molecular mass of 16,886 Da.
Reconstitution	It is recommended to reconstitute the lyophilized rHuTNF-alpha mutant in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Formulation	Lyophilized from a 0.2 um filtered solution in PBS
Molecular Weight	16,886 Da
Source	E. coli
Host	Human
Endotoxin Level	Less than 0.01 ng/ug (0.1 EU/ug) determined by LAL test
Bioactivity	The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.01 ng/ml, corresponding to a Specific Activity of 1.0x108 IU/mg.

Synonyms	Tumor Necrosis Factor alpha (TNF)-? (mutant), human; TNF-alpha mutant, human
Sequence	Analysis?The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Lys-Arg-Lys.

Purity	Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Anion-exchange FPLC.(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Storage	Lyophilized samples are stable for up to twelve months from date of receipt at -20oC to -70°C. Please avoid repeated freeze-thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500006-1	IX00404-1	1mg	$1747		Add to Cart
BHP18500006-2	IX00404-10	10ug	$107		Add to Cart
BHP18500006-3	IX00404-50	50ug	$295		Add to Cart

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