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Home / Proteins & Peptide

TNF-alpha, His, Mouse

SKU : BHP18500021

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iXCells Biotechnologies

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Mfr.No.	IX02684, IX02684
Description	Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-alpha) produced in E. coli is a non-glycosylated polypeptide chain of 163 amino acids. A fully biologically active molecule, rhTNF-alpha has a molecular mass of 18.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques.
Reconstitution	Reconstituted in ddH2O at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against PBS.
Molecular Weight	18.3kDa, observed by reducing SDS-PAGE.
Source	E. coli
Host	Mouse
Endotoxin Level	1 EU/ug, determined by LAL method.
Bioactivity	ED500.05ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of?2x 107units/mg.

Synonyms	Tumor Necrosis Factor-alpha (TNF-alpha), Cachectin.
Sequence	MHHHHHHMLR SSSQNSSDKP VAHVVANHQV EEQLEWLSQRANALLANGMD LKDNQLVVPA DGLYLVYSQV LFKGQGCPDYVLLTHTVSRF AISYQEKVNL LSAVKSPCPK DTPEGAELKPWYEPIYLGGV FQLEKGDQLS AEVNLPKYLD FAESGQVYFGVIAL

Purity	95% as analyzed by reducing SDS-PAGE.
Storage	Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-slpha) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rmTNF-alpha should be stable up to 4 week at 4°C or up to 6 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500021-1	IX02684	100ug	$324		Add to Cart

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