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Home / Proteins & Peptide

TEV Protease, His

SKU : BHP18500225

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iXCells Biotechnologies

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Mfr.No.	IX03030, IX03030-10K, IX03030-1K
Description	Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the tags from fusion proteins. The optimal temperature for cleavage is 30°C; also it can be used at temperature as low as 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) (ENLYFQ(G/S)) and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques.
Formulation	Sterile liquid solution contains 50mM Tris, 5mM DTT, 50% glycerol, pH7.5.
Molecular Weight	28.4 kDa, observed by reducing SDS-PAGE.
Source	E. coli
Notes	For research use only
Bioactivity	10 IU/ul. Unit Definition: One unit of TEV protease cleaves?85% of 3 ug of control substrate in 1 hour at pH 8.0 at 30°C.

Synonyms	rTEV, TEV, P1 protease

Purity	95% by SDS-PAGE analyses.
Storage	Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20°C from date of receipt. Please avoid freeze-thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500225-1	IX03030-10K	10000IU	$263		Add to Cart
BHP18500225-2	IX03030-1K	1000IU	$49		Add to Cart

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