Human Ten-eleven translocation 3 protein (TET3), also known as methylcytosine dioxygenase TET3, GenBank Accession # NM_001287491, a.a. 824-1795 (end) with N-terminal FLAG-tag and C-terminal His-tag. MW = 108 kDa, expressed in Sf9 cells via a baculovirus expression system.
Host cell line: Sf9 cells
Immunogen Region
824-1795 (end)
Tag
N-terminal FLAG-tag, C terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, and 140 mM Imidazole
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Avoid freeze/thaw cycles.
GenBank
NM_001287491
Mw(kda)
108 kDa
Synonyms
methylcytosine dioxygenase TET3
Uniprot
O43151
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Reference
1. Tian-Peng, G., et al., Nature. 2011;477: 606-610.2. Abdel-Wahab, O., et al., Blood. 2009; 114: 144-147.