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Home / Proteins & Peptide

TET2, FLAG-His-tag

SKU : BHP18200360

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Mfr.No.	50162
Description	Human Ten-eleven translocation 2 protein (TET2), methylcytosine dioxygenase TET2, GenBank Accession # NM_001127208, a.a. 1129-2002 (end) with N-terminal FLAG-tag, C-terminal His-tag. MW = 100 kDa, expressed in Sf9 cells via a baculovirus expression system. Host cell line: Sf9 cells
Immunogen Region	1129-2002
Tag	N-terminal FLAG-tag, C terminal His tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Add 50 ul reaction mix containing a-ketoglutarate, TET enzyme, ATP, and TET SA assay buffer (BPS) to wells coated with the substrate. TET SA buffer contains saturable concentrations of iron, a-KG, and ATP. Incubate 3hrs at 37°C. Add antibodyagainst hydroxymethylated cytosine, incubate 1 hr. Then add secondary HRP labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence. Avoid freeze/thaw cycles.
GenBank	NM_001127208
Mw(kda)	100 kDa

Synonyms	Ten-eleven translocation 2 protein, methylcytosine dioxygenase TET2
Uniprot	Q6N021

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Avoid freeze/thaw cycles.

Reference	1. Myunggon, K., et al., Nature. 2013;497: 122-126.2. Shinsuke, I, et al., Nature. 2010; 466: 1129-1133.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200360	50162	50 ug	$533.50		Add to Cart

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