Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits and encompassing amino acids 16-1213. This protein contains three mutations: K417T, E484K and N501Y that have been found in emerging SARS-CoV-2 Variants of Concern and may lead to higher transmissibility and infectivity. This mutant Spike Trimer will be useful for structure-function studies, testing of neutralizing antibodies, or antibody and drug screening. The construct also contains a C-terminal His-tag. Note that the expected MW of the S1+S2 monomer is 136kDa but migrates at a higher MW in SDS-PAGE due to glycosylation. The recombinant protein is 90% pure following affinity purification.
Formulation
8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
Species
SARS-CoV-2
Notes
Avoid freeze/thaw cycles.
GenBank
MN908947
Mw(kda)
137 kDa +glycans
Synonyms
coronavirus, covid, covid-19, covid19, Sars-cov-2 p1, sars cov 2 Spike, sarscov2, SARS-CoV-2 variant b11281, SARS-CoV-2 variant b1135, K417T mutation, Brazil coronavirus, SARS-CoV-2 Brazil, B1135 variant, South Africa variant, 100988-1, 100988-2