Severe acute respiratory Coronavirus 2 Spike Glycoprotein S1 (SARS-CoV-2 Spike S1), GenBank Accession No. QHD43416.1, a.a. 16-685, fused at the C-terminus of the Fc portion of human IgG1, with a C-terminal Avi-tag, expressed in a HEK293 expression system enzymatically biotinylated using Avi-tag technology. Biotinylation is confirmed to be>=90%. MW=104 kDa. This protein runs at a higher molecular weight due to glycosylation.
Immunogen Region
16-685
Tag
C-terminal Fc-Avi-tag
Formulation
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
SARS-CoV-2
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
SARS-CoV-2 S1(Biohippo #100720) and ACE2 binding assay was done using BPS ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit (Biohippo #79936). SARS-CoV-2 S1 (Biohippo #100720) protein and Streptavidin-HRP were used instead of SARS-CoV-2 Spike protein (RBD) and anti-mouse Fc-HRP respectively.