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Home / Proteins & Peptide

Spike RBD (B.1.351 Variant) Avi-His-Tag (SARS-CoV-2)

SKU : BHP18201325

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BPS Bioscience

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Mfr.No.	100978-2
Description	Recombinant SARS-CoV-2 Spike protein, RBD (Receptor Binding Domain) encompassing amino acids 319-541. This protein corresponds to SARS-CoV-2 Variant B.1.351, orignally discovered in South Africa and contains mutations K417N, E484K and N501Y. It also contains a C-terminal Avi-Tag and a C-terminal His-tag. The recombinant protein is>=90% pure following high affinity Ni-NTA purification and shows less than 5% aggregation in gel filtration. Constructs: Spike RBD (B.1.351) (SARS-CoV-2) (319-541-Avi-His)
Immunogen Region	319-541
Tag	C-terminal Avi-His-Tag
Formulation	8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
Mutation	K417N, E484K, N501Y
Species	SARS-CoV-2
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was done according to the Spike RBD (B.1.1.7 Variant) (N501Y) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit (Biohippo #78140) with various amounts of ACE2, His-Avi-Tag, Biotinlabeled HiP (Biohippo #100665). Avoid freeze/thaw cycles.
GenBank	MN908947
Mw(kda)	28 kDa

Synonyms	coronavirus, covid, covid-19, covid19, Sars-cov-2 B1351, 20H/501Y.V2, sars cov 2 Spike Trimer, sarscov2, South African Variant, B1351 mutation, South Africa coronavirus, SARS-CoV-2 S. African variant, 100978-1, 100978-2
Uniprot	P0DTD1

Shipping Temperature	-80°C (dry ice)
Purity	>=90%
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201325	100978-2	1 mg	$3135		Add to Cart

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