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Home / Proteins & Peptide

Sortase A Pentamutant, S. aureus, His-tag

SKU : BHP18200559

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Mfr.No.	71046
Description	Staphylococcus aureus sortase A Pentamutant (P94R, D160N, D165A, K190E, K196T), a.a. 26-206(end) GenBank Accession No. AF162687 with N-terminal His tag, MW = 21.7 kDa, expressed in an E. coli expression system.
Background	Staphylococcal Sortase A is a bacterial transpeptidase that covalently attaches proteins to the bacterial cell wall by cleaving between threonine and glycine at an LPXTG recognition motif and catalyzes the formation of an amide bond between the carboxyl-group of threonine and the amino-group of the cell-wall peptidoglycan. This chemistry has been exploited as a molecular "stapler" to site-specifically link proteins with the C-terminal LPETGX motif to other proteins or molecules possessing a glycine or aminomethylene motif. The Sortase A Pentamutant exhibits higher activity than the wild type protein. This reaction is applicable to proteins in solution and on the living cell surface.
Immunogen Region	26-206(end)
Tag	N-terminal His-tag
Formulation	40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 400 mM imidazole and 20% glycerol
Species	S. aureus
Application	Suitable for use with in vitro protein ligation reactions.
Notes	Assay was done in the reaction buffer (50 ul) containing 50 mM HEPES (pH=7.4), 150 mM NaCl, 5 mM CaCl2, 5 mM (Gly)3, 25 mM Abz/Dnp substrate, and Sortase A for 30 min at 30°C. Fluorescence intensity was measured at Ex320nm/Em420nm. Avoidfreeze/thaw cycles. Storing diluted enzymeis not recommended, if necessary, usecarrier protein (BSA 0.1 - 0.5%).
GenBank	AF162687
Mw(kda)	21.7 kDa

Synonyms	SrtA, sortase A, pentamutant
Uniprot	C7TEJ5

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Chen, I. et al., Proc Natl. Acad. Sci. (USA). 2011; 108(28): 11399-11404. 2. Kappel, K., et al., Protein Sci. 2012 Dec;21(12):1858-71.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200559	71046	50 ug	$566.50		Add to Cart

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