SIRP alpha protein, or Signal regulatory proteinalpha (SIRP-alpha), also known as Tyrosine protein phosphatase non-receptor type substrate 1, CD172a, Bit, and MyD-1, GenBank Accession No. NM_080792, a.a. 27-370, with C-terminal Avi and His tags, MW 40.3 kDa*, expressed in a HEK293 expression system and enzymatically biotinylated using Avi-tag technology. This SIRP alpha protein acts as a receptor for CD47; a.a. 27-370 correspond to the extracellular portion of SIRP-alpha. *Note: SIRP-alpha is heavily glycosylated, resulting in higher molecular weight.
Immunogen Region
27-370
Tag
C-terminal His-Avi-Tags
Formulation
8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
HiP indicates a high purity protein with>=90% purity as measured by gel filtration. Avoid freeze/thaw cycles.
GenBank
NM_080792
Mw(kda)
40.3 kDa
Synonyms
SIRP alpha protein, sirpa protein, sirp-a protein, SIRPalpha, CD172a, Tyrosine-protein phosphatase non-receptor type substrate 1, CD172a, Bit, and MyD-1
Uniprot
P78324
Shipping Temperature
-80°C (dry ice)
Storage
At least 6 months at -80°C.
Reference
1. Hatherley, D., et al., Mol. Cell, 2008, 31:266-77 2. Baba, N., et al., J. Exp. Med., 2013, 210(6):1251-63