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Home / Proteins & Peptide

Shh, Mouse(CHO-expressed)

SKU : BHP18500211

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iXCells Biotechnologies

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Mfr.No.	IX03008, IX03008-1, IX03008-10, IX03008-50
Description	Members of the Hedgehog (Hh) family are highly conserved proteins which are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). The biologically active form of Hh molecules is obtained by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal one half of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can substitute for each other in experimental systems. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.
Reconstitution	Reconstituted in ddH2O or PBS at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against PBS.
Molecular Weight	20 kDa, observed by non-reducing SDS-PAGE.
Source	CHO
Host	Mouse
Endotoxin Level	0.2 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	ED50 1 ug/ml, measured by its ability to induce alkaline phosphatase production by CCL-226 cells, corresponding to a specific activity of 1 x 103 units/mg.

Synonyms	SHH, HHG-1
Sequence	CGPGRGFGKR RHPKKLTPLA YKQFIPNVAE KTLGASGRYEGKITRNSERF KELTPNYNPD IIFKDEENTG ADRLMTQRCKDKLNALAISV MNQWPGVKLR VTEGWDEDGH HSEESLHYEGRAVDITTSDR DRSKYGMLAR LAVEAGFDWV YYESKAHIHCSVKAENSVAA KSGG

Purity	95% as analyzed by SDS-PAGE and HPLC.
Storage	Lyophilized recombinant Murine Sonic Hedgehog (SHH) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmSHH should be stable up to 1 week at 4°C or up to 2 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500211-1	IX03008-1	1mg	$1498		Add to Cart
BHP18500211-2	IX03008-10	10ug	$82		Add to Cart
BHP18500211-3	IX03008-50	50ug	$199		Add to Cart

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