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Home / Proteins & Peptide

Set7/Set9, GST-tag (E. coli-derived)

SKU : BHP18200409

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BPS Bioscience

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Mfr.No.	51010
Description	Human recombinant SET domain containing lysine methyltransferase 7 (SETD7), or SET7/9 (GenBank Accession No. NM_030648), a.a. 52-266 (end) with N-terminal GST-tag, MW = 61 kDa, expressed in an E. coli expression system. Specific activity: 0.58 pmol/min/ug
Background	SETD7 or SET domain containing lysine methyltransferase 7 is a lysine methyltransferase which can methylates lysine-4 (K4) in histone H3 in vitro and in vivo. Methylation of K4 in histone H3 by SETD7 and methylation of K9 in histone H3 by SUV39H1 were found to have differential effects on subsequent histone acetylation by p300. SETD7 can also methylate p53 at lys372 within the C-terminal regulatory region. Methylated p53 is restricted to the nucleus and the modification positively affects its stability. SETD7 regulates the expression of p53 target genes in a manner dependent on the p53 methylation site.
Immunogen Region	52-266 (end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 20 uM S-adenosyl-methionine, and 0-10 ng SET7/9) add to the wells coated with the substrate. Incubate at room temp for 1 hr. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then incubate 30 mins with HRP-labeled secondary antibody. Finally, add HRP chemiluminescent substrates and read luminescence. Avoid freeze/thaw cycles.
GenBank	NM_030648
Mw(kda)	61 kDa

Synonyms	Set7/9, SETD7, KIAA1717, KMT7, SET7, SET9
Uniprot	Q8WTS6

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Ivanov G.S. et al., Mol. Cell. Biol. 27 (19), 6756-6769 (2007). 2. Couture J.F. et al., Nat. Struct. Mol. Biol. 13 (2), 140-146 (2006).Application Reference(s): Selective inhibitors of bacterial t-RNA-(N(1)G37) methyltransferase (TrmD) that demonstrate novel ordering of the lid domain (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200409	51010	100 ug	$357.50		Add to Cart

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