SUMO Protease otherwise known as Ulp1, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae. This highly specific protease cleaves off sumo tags by recognizing the tertiary structure of SUMO as opposed to recognizing its amino acid sequence. Because of its recognition of SUMOs tertiary structure, it has little to no non-specific proteolysis keeping your protein samples safe after all affinity tags have been cleaved off. SUMO protease is active from 2°C to 37°C in a P.H range of 7.0 9.0, for lower temperatures allow more time for the cleavage reaction.
Product Type
Recombinant Proteins
Tag
His
Field of Use
Enzymes
Molecular Weight
27.4 kDa
Application
Recombinant protein SUMO tag removal, recombinant protein characterization, ELISA, Western blot, crystallization studies.
Expression Source
E. coli
Uniprot
QHB12236.1 (Genbank)
Shipping
Dry Ice
Purity
>90%, SDS-PAGE
Storage
Stable for 12 months at -80°C, Avoid freeze/thaw cycles