Please refer to the printed manual for detailed information.
Background
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Reactivity
SARS
Tag
C-His
Description
A DNA sequence encoding the S1 subunit of SARS-CoV (isolate:WH20) spike (AAX16192.1) (Met1-Arg667) was expressed with a C-terminal polyhistidine tag.
Formulation
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Host
Baculovirus-Insect Cells
Accession Number
AAX16192.1
Endotoxin Level
< 1.0 EU per ?g of the protein as determined by the LAL method
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(PKSH031870) at 2ug/mL (100uL/well) can bind Recombinant SARS-CoV S1 Protein (His Tag)?Active?(PKSV030101),the EC50 of SARS-CoV S1 Protein (His Tag)?Active?(PKSV030101) is 400-850 ng/mL.
Mol Weight
74.4kDa
Mw(kda)
74.4kDa
Sequence Info
Met1-Arg667
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with ice bag. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.