Please refer to the printed manual for detailed information.
Background
Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).
Reactivity
Rat
Tag
C-His
Description
A DNA sequence encoding the extracellular domain of rat RBP4 (NP_037294.1) (Met 1-Leu 201) was expressed, fused with a polyhistidine tag at the C-terminus.
Formulation
Lyophilized from sterile PBS, pH 7.4
Host
HEK293 Cells
Accession Number
NP_037294.1
Endotoxin Level
< 1.0 EU per ?g of the protein as determined by the LAL method
Bioactivity
Measured by its ability to bind alltrans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4.The 50% binding concentration (BC50) is >0.5 µM.
Mol Weight
22.8 kDa
Mw(kda)
22.8 kDa
Sequence Info
Met 1-Leu 201
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.