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Home / Proteins & Peptide

Recombinant Rat Leptin

BHP11002388
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Email
orders@biohippo.com
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Uniprot P50596
Shipping Shipped at Room temp
Description Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Solubility: It is recommended to reconstitute the lyophilized Leptin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of Leptin rat as a Reference Standard.
Format Sterile Filtered White lyophilized (freeze-dried) powder.
Purity Greater than 95.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.
Expiry Date Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Reference Title: Expression of Hypothalamic KiSS-1 System and Rescue of Defective Gonadotropic Responses by Kisspeptin in Streptozotocin-Induced Diabetic Male Rats.
Publication: doi: 10.2337/db05-1584 Diabetes September 2006 vol. 55 no. 9 2602-2610

Title: Desensitization of gonadotropin responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states.
Publication: Am J Physiol Endocrinol Metab 294: E1088–E1096, 2008.294: E1088–E1096, 2008. First published April 15, 2008; doi:10.1152/ajpendo.90240.2008.

Title: p38 MAPK and LXR? mediate leptin effect on SREBP-1c expression in hepatic stellate cells (short
Publication:

Title: Neuroprotection by leptin in a rat model of permanent cerebral ischemia: effects on STAT3 phosphorylation in discrete cells of the brain.
Publication: Citation: Cell Death and Disease (2011) 2, e238; doi:10.1038/cddis.2011.125Published online 8 December 2011.

Title: Leptin effect on SREBP-1c expression) .
Publication:

Title: The mammalian target of rapamycin as novel central regulator of puberty onset via modulation of hypothalamic Kiss1 system.
Publication: Endocrinology 150.11 (2009): 5016-5026.Link http://press.endocrine.org/doi/full/10.1210/en.2009-0096
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BHP11002388 cyt-227 200ug $60
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BHP11002388 cyt-227 1mg $145
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BHP11002388 cyt-227 5mg $450
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