Please refer to the printed manual for detailed information.
Background
Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD130/gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130/gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. CD130/gp130 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD130/gp130 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.
Reactivity
Rat
Tag
C-His
Description
A DNA sequence encoding the rat IL6ST (NP_001008725.2) extracellular domain (Met 4-Glu 618) was fused with a polyhistidine tag at the C-terminus.
Formulation
Lyophilized from sterile PBS, pH 7.4
Host
HEK293 Cells
Accession Number
NP_001008725.2
Endotoxin Level
< 1.0 EU per ?g of the protein as determined by the LAL method
Bioactivity
Measured by its ability to inhibit the IL6 Ralpha enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168.). The ED50 for this effect is typically 0.5-2 ug/ml in the presence of 50 ng/ml recombinant human IL6sR and 100 ng/ml recombinant human IL6.
Mol Weight
68.2 kDa
Mw(kda)
68.2 kDa
Sequence Info
Met 4-Glu 618
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.