TGF-beta receptor type-2; TGFR-2; TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II; Tgfbr2
Background
Transforming growth factor-beta (TGF-beta) is an essential regulator in the processes of development, cell proliferation, and extracellular matrix deposition. TGF-beta regulates cellular processes by binding to three high-affinity cell surface receptors: TGF-beta receptor type I (TGF-beta-RI), TGF-beta receptor type II (TGF-beta-RII), and TGF-betabeta receptor type III (TGF-beta-RIII). TGF-beta RII is consists of a C-terminal protein kinase domain and an N-terminal ectodomain and belongs to transforming growth factor-beta (TGF-beta) receptor subfamily. TGF-beta RII has a protein kinase domain which can form a heterodimeric complex with another receptor protein and bind TGF-beta. This receptor/ligand complex phosphorylates protein will enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation.
Biological Activity
Measured by its ability to inhibit TGF-beta 1 activity on TF?1 human erythroleukemic cells.The ED50 for this effect is 1680.39ng/ml in the presence of 1ng/ml of recombinant human TGF-beta 1.
Description
Recombinant Mouse Transforming Growth Factor-beta Receptor Type II is produced by our Mammalian expression system and the target gene encoding Ile24-Asp159 is expressed with a 6His tag at the C-terminus.
Molecular Weight
16.2 KDa
Accession Number
Q62312-2
Endotoxin Level
Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Notes
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100ug/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Purity
Greater than 95%
Storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.