Please refer to the printed manual for detailed information.
Background
Urokinase plasminogen activator (uPA) and/or its receptor (uPAR) are essential for metastasis, and overexpression of these molecules is strongly correlated with poor prognosis in a variety of malignant tumours. uPAR and uPA levels in both resected tumor tissue and plasma are of independent prognostic significance for patient survival in several types of human cancer. This system has classically been thought to drive tumor progression by mediating directed extracellular proteolysis on the surface of migrating or invading cells, and intervening with this proteolysis by targeting uPAR has been proposed to represent a novel approach for inhibiting tumor progression. uPAR, also known as PLAUR or CD87, has been implicated in the growth, metastasis, and angiogenesis of several solid and hemotologic malignancies. uPAR is a highly glycosylated, 55-60kDa integral membrane protein linked to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. It is part of a cell surface system that also consists of the serine protease uPA and several specific inhibitors (plasminogen activator inhibitors 1 and 2). Additionally, the analysis of CD87 (urokinase-type plasminogen activator receptor - uPAR) expression has a potential role in the diagnostic or prognostic work-up of several hematological malignancies, particularly acute leukemia and multiple myeloma.
Reactivity
Mouse
Tag
C-His-Fc
Description
A DNA sequence encoding the extracellular domain (Met 1-Thr 297) of mouse PLAUR (NP_035243.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Formulation
Lyophilized from sterile PBS, pH 7.4
Host
HEK293 Cells
Accession Number
NP_035243.1
Endotoxin Level
< 1.0 EU per ?g of the protein as determined by the LAL method.
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized human uPA at 5 ug/ml (100 ul/well) can bind mouse PLAUR with a linear ranger of 1.6-40 ng/ml.
Mol Weight
58 kDa
Mw(kda)
58 kDa
Sequence Info
Met 1-Thr 297
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.