Please refer to the printed manual for detailed information.
Background
Polymeric immunoglobulin receptor, also known as PIGR, is a member of the immunoglobulin superfamily and a Fc receptor. The ectodomain of this receptor consists of five units with homology to the variable units of immunoglobulins and a transmembrane region, which also has some homology to certain immunoglobulin variable regions. PIGR is expressed on several glandular epithelia including those of liver and breast. The deduced amino-acid sequence has a length of 764 residues and shows an overall similarity of 56% and 64% with the rabbit and rat counterpart. PIGR mediates transcellular transport of polymeric immunoglobulin molecules, and thus facilitates the secretion of IgA and IgM. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment of PIGR.
Reactivity
Mouse
Tag
C-His
Description
A DNA sequence encoding the extracellular domain (Met 1-Lys 645) of mouse PIGR (NP_035212.2) precursor was expressed with a C-terminal polyhistidine tag.
Formulation
Lyophilized from sterile PBS, pH 7.4
Host
HEK293 Cells
Accession Number
NP_035212.2
Endotoxin Level
< 1.0 EU per ?g of the protein as determined by the LAL method.
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PIGR at 5 ug/ml (100 ul/well) can bind mouse IgM with a linear range of 0.156-10 ug/ml.
Mol Weight
70.8 kDa
Mw(kda)
70.8 kDa
Sequence Info
Met 1-Lys 645
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.