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Home / Proteins & Peptide

Recombinant Mouse IL3

SKU : BHP10900740

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Z200145
Description	IL3 is produced mainly by T cells following cell activation by antigens and mitogens, but also by keratinocytes, natural killer cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial-derived recombinant IL3 shows that glycosylation is not required for the activity of IL3. IL-3 sequences are evolutionarily less well conserved. Human and murine IL3 show approximately 29% homology at the protein level while murine and rat IL3 show approximately 54% homology. IL3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells, and various myeloid leukemia cells. Binding of IL3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein. Recombinant mouse IL3 is a monomer of 15 kDa.
Alternative Names	IL-3 ; Mast cell growth factor ; MCGF ; Multipotential colony-stimulating factor ; Multi-CSF ; HCGF ; P-cell stimulation factor ; Hematopoietic growth factor
Quantity	10 µg
Regulatory	RUO
Source	E. coli
Host	Mouse
Endotoxin Level	<1.0 EU/μg of recombinant protein as determined by the LAL method
Biological Activity Comment	The ED50 as determined by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells was found to be in the <0.05 ng/mL

Weight	15.0 kDa

Description	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Format	Lyophilized Powder Lyophilized from a 0.2 μm filtered solution in PBS
Purity	>95% as determined by SDS-PAGE
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Immunogen Species	Mus Musculus (Mouse)

MSDS	Growth Factors, Cytokines & Kinases MSDS
Other	Growth Factor and Cytokine Brochure

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SKU	Supplier No.	Size	Your price	Quantity
BHP10900740	Z200145	10µg	$169.44		Add to Cart

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