Glioma pathogenesis-related protein 1, also known as Protein RTVP-1, GLIPR1 and GLIPR, is a single-pass membrane protein which belongs to the CRISP family. GLIPR1/ RTVP-1 was expressed in high levels in glioblastomas, whereas its expression in low-grade astrocytomas and normal brains was very low. Transfection of glioma cells with small interfering RNAs targeting GLIPR1/ RTVP-1 decreased cell proliferation in all the cell lines examined and induced cell apoptosis in some of them. Overexpression of GLIPR1/ RTVP-1 increased astrocyte and glioma cell proliferation and the anchorage-independent growth of the cells. In addition, overexpression of GLIPR1/ RTVP-1 rendered glioma cells more resistant to the apoptotic effect of tumor necrosis factor-related apoptosis-inducing ligand and serum deprivation. GLIPR1/ RTVP-1 regulated the invasion of glioma cells was evident by their enhanced migration through Matrigel and by their increased invasion in a spheroid confrontation assay. The increased invasive potential of the GLIPR1/ RTVP-1 overexpressors was also shown by the increased activity of matrix metalloproteinase 2 in these cells. The expression of GLIPR1/ RTVP-1 is correlated with the degree of malignancy of astrocytic tumors and that GLIPR1/ RTVP-1 is involved in the regulation of the growth, survival, and invasion of glioma cells. GLIPR1/ RTVP-1 is a potential therapeutic target in gliomas.
Tag
His tag
Description
A DNA sequence encoding the mouse GLIPR1 (NP_082884.1) (Met1-Thr223) was expressed with a C-terminal polyhistidine tag.
Regulatory
RUO
Host
Human Cells
Endotoxin Level
< 1.0 EU per μg of the protein as determined by the LAL method
Nature
Recombinant
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Buffer
Lyophilized from sterile PBS, pH 7.4
Description
Please refer to the printed manual for detailed information.
Purity
> 85 % as determined by SDS-PAGE
Expiry Date
Samples are stable for up to twelve months from date of receipt at -70℃
Immunogen Species
Mus Musculus (Mouse)
Attachment
Reviews of Recombinant Mouse GLIPR1 Protein (His Tag)