Please refer to the printed manual for detailed information.
Background
Endoglin, also known as CD105, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-beta superfamily ligands, endoglin binds TGF-beta1 and TGF-beta3 with high affinity not by itself but by associating with TGF-beta type I I receptor (TbetaRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.
Reactivity
Mouse
Tag
C-His
Description
A DNA sequence encoding the mouse CD105 (NP_031958.2) extracellular domain (Met 1-Gly 580) was expressed, with a C-terminal polyhistidine tag.
Formulation
Lyophilized from sterile PBS, pH 7.4
Host
HEK293 Cells
Accession Number
NP_031958.2
Endotoxin Level
< 1.0 EU per ?g of the protein as determined by the LAL method.
Bioactivity
1. Measured by its ability to bind Human ENG-Fc in functional Elisa.2. Measured by its ability to latent TGFB1-His in functional Elisa.3. Measured by its ability to mouse ENG-His in functional Elisa.4. Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953. The ED50 for this effect is typically 5-15 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.
Mol Weight
61.2 kDa
Mw(kda)
61.2 kDa
Sequence Info
Met 1-Gly 580
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.