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Home / Proteins & Peptide

Recombinant Mouse CSK/C-Src kinase Protein (Active)

SKU : BHP10305072

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSM040451
Alternative Names	AW212630;p50CSK
Reconstitution	Please refer to the printed manual for detailed information.
Background	The tyrosine kinase c-Src has been implicated as a modulator of cell proliferation, spreading, and migration. These functions are also regulated by Met. The structure of a large fragment of the c-Src kinase comprises the regulatory and kinase domains and the carboxy-terminal tall. c-Src kinase interactions among domains and is stabilized by binding of the phosphorylated tail to the SH2 domain. This molecule is locked in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase. The protein-tyrosine kinase activity of c-Src kinase is inhibited by phosphorylation of tyr527, within the c-Src c-terminal tail. Genetic and biochemical data have suggested that this negative regulation requires an intact Src homology 2 (SH2) domain. Since SH2 domains recognize phosphotyrosine, it is possible that these two non-catalytic domains associate, and thereby repress c-Src kinase activity. Experiments have suggested that c-Src kinase plays a role in the biological behaviour of colonic carcinoma cells induced by migratory factors such as EGF, perhaps acting in conjunction with FAK to regulate focal adhesion turnover and tumour cell motility. Furthermore, although c-Src kinase has been implicated in colonic tumour progression, in the adenoma to carcinoma in vitro model c-Src is not the driving force for this progression but co-operates with other molecules in carcinoma development. References
Reactivity	Mouse
Description	A DNA sequence encoding the mouse CSK (P41241?) (Met 1-Leu 450) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
Host	Baculovirus-Insect Cells
Accession Number	P41241
Endotoxin Level	< 1.0 EU per ?g of the protein as determined by the LAL method.
Bioactivity	Kinase activity untested
Mol Weight	50.9 kDa
Mw(kda)	50.9 kDa

Sequence Info	Met 1-Leu 450

Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note	Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity	> 90 % as determined by SDS-PAGE

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10305072	PKSM040451	50ug	$634		Add to Cart

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