Please refer to the printed manual for detailed information.
Background
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
Reactivity
Mouse
Tag
C-His
Description
A DNA sequence encoding the mouse CA8 (P28651) (Met 1-Gln 291) was expressed, with a C-terminal polyhistidine tag.
Formulation
Lyophilized from sterile 50mM Tris, pH 8.0
Host
E.coli
Accession Number
P28651
Endotoxin Level
Please contact us for more information.
Bioactivity
Measured by its esterase activity. The specific activity is >5 pmoles/min/ug.
Mol Weight
34.5 kDa
Mw(kda)
34.5 kDa
Sequence Info
Met 1-Gln 291
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.